Co-occurrence of nitrite-dependent anaerobic methane oxidizing andanaerobic ammonia oxidizing bacteria in two Qinghai-Tibetan saline lakes

ISL OpenIR > 青海盐湖研究所知识仓储

	Co-occurrence of nitrite-dependent anaerobic methane oxidizing andanaerobic ammonia oxidizing bacteria in two Qinghai-Tibetan saline lakes
	Yang, Jian; Jiang, Hongchen; Wu, Geng; Hou, Weiguo; Sun, Yongjuan; Lai, Zhongping; Dong, Hailiang
	2012
发表期刊	FRONTIERS OF EARTH SCIENCE
ISSN	2095-0195
卷号	6 期号:4 页码:383-391
关键词	Anammox N-damo Co-occurrence Saline Lakes Qinghai-tibetan Plateau
摘要	Nitrite-dependent anaerobic methane-oxidizing (n-damo) bacteria and anaerobic ammonia oxidizing (anammox) bacteria are two groups of microorganisms involved in global carbon and nitrogen cycling. In order to test whether the n-damo and anammox bacteria co-occur in natural saline environments, the DNA and cDNA samples obtained from the surficial sediments of two saline lakes (with salinity of 32 and 84 g/L, respectively) on the Tibetan Plateau were PCR-amplified with the use of anammox- and n-damo-specific primer sets, followed by clone library construction and phylogenetic analysis. DNA and cDNA-based clones affiliated with n-damo and anammox bacteria were successfully retrieved from the two samples, indicating that these two groups of bacteria can co-occur in natural saline environments with salinity as high as 84 g/L. Our finding has great implications for our understanding of the global carbon and nitrogen cycle in nature. Nitrite-dependent anaerobic methane-oxidizing (n-damo) bacteria and anaerobic ammonia oxidizing (anammox) bacteria are two groups of microorganisms involved in global carbon and nitrogen cycling. In order to test whether the n-damo and anammox bacteria co-occur in natural saline environments, the DNA and cDNA samples obtained from the surficial sediments of two saline lakes (with salinity of 32 and 84 g/L, respectively) on the Tibetan Plateau were PCR-amplified with the use of anammox- and n-damo-specific primer sets, followed by clone library construction and phylogenetic analysis. DNA and cDNA-based clones affiliated with n-damo and anammox bacteria were successfully retrieved from the two samples, indicating that these two groups of bacteria can co-occur in natural saline environments with salinity as high as 84 g/L. Our finding has great implications for our understanding of the global carbon and nitrogen cycle in nature.
语种	英语
文献类型	期刊论文
条目标识符	http://ir.isl.ac.cn/handle/363002/2015
专题	青海盐湖研究所知识仓储
推荐引用方式 GB/T 7714	Yang, Jian,Jiang, Hongchen,Wu, Geng,et al. Co-occurrence of nitrite-dependent anaerobic methane oxidizing andanaerobic ammonia oxidizing bacteria in two Qinghai-Tibetan saline lakes[J]. FRONTIERS OF EARTH SCIENCE,2012,6(4):383-391.
APA	Yang, Jian.,Jiang, Hongchen.,Wu, Geng.,Hou, Weiguo.,Sun, Yongjuan.,...&Dong, Hailiang.(2012).Co-occurrence of nitrite-dependent anaerobic methane oxidizing andanaerobic ammonia oxidizing bacteria in two Qinghai-Tibetan saline lakes.FRONTIERS OF EARTH SCIENCE,6(4),383-391.
MLA	Yang, Jian,et al."Co-occurrence of nitrite-dependent anaerobic methane oxidizing andanaerobic ammonia oxidizing bacteria in two Qinghai-Tibetan saline lakes".FRONTIERS OF EARTH SCIENCE 6.4(2012):383-391.