A fluorescein- based chemosensor for relay fluorescence recognition of Cu(II) ions and biothiols in water and its applications to a molecular logic gate and living cell imaging

ISL OpenIR > 青海盐湖研究所知识仓储

	A fluorescein- based chemosensor for relay fluorescence recognition of Cu(II) ions and biothiols in water and its applications to a molecular logic gate and living cell imaging
	Fu, Zhen-Hai1,2 ; Yan, Lu-Bin 3; Zhang, Xiaolong 1; Zhu, Fan-Fan 1; Han, Xin-Long 1; Fang, Jianguo 1; Wang, Ya-Wen 1; Peng, Yu 1
	2017-05-21
发表期刊	ORGANIC & BIOMOLECULAR CHEMISTRY
卷号	15 期号:19 页码:4115-4121
摘要	Relay recognition of copper(II) ions and biothiols via a fluorescence "on off on" cascade was designed and realized as a new sequential combination of cations and small molecules. Probe 1 bearing a fluorescein skeleton was thus synthesized, which performed well in 100% HEPES buffer (pH = 7.0) solution, as a highly sensitive, selective fluorescence sensor for Cu2+. The limit of detection (LOD, 0.017 ppm) was obtained, and this value is much lower than 1.3 ppm, allowed by US EPA. The 1 : 1 complex generated from fast sensing of Cu2+ when excited at 491 nm, showed good relay recognition for biothiols (i.e., Cys, Hcy and GSH with low detection limits of 0.12 mu M, 0.036 mu M and 0.024 mu M, respectively) via remarkable fluorescence enhancement. The origin of this relay process was disclosed through ESI-MS and corresponding density functional theory (DFT) computations. Notably, probe 1 can be utilized for the construction of a molecular logic gate with the IMPLICATION function by using the above fluorescence changes. Moreover, this relay recognition was also applied to HepG2 cell imaging successfully.
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.isl.ac.cn/handle/363002/4242
专题	青海盐湖研究所知识仓储
作者单位	1.Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Qinghai Inst Salt Lakes, Key Lab Salt Lakes Resources & Chem, Xining 810008, Peoples R China 3.Sun Yat Sen Univ, Affiliated Hosp 6, Dept Pediat Surg, Guangzhou 510655, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Fu, Zhen-Hai,Yan, Lu-Bin,Zhang, Xiaolong,et al. A fluorescein- based chemosensor for relay fluorescence recognition of Cu(II) ions and biothiols in water and its applications to a molecular logic gate and living cell imaging[J]. ORGANIC & BIOMOLECULAR CHEMISTRY,2017,15(19):4115-4121.
APA	Fu, Zhen-Hai.,Yan, Lu-Bin.,Zhang, Xiaolong.,Zhu, Fan-Fan.,Han, Xin-Long.,...&Peng, Yu.(2017).A fluorescein- based chemosensor for relay fluorescence recognition of Cu(II) ions and biothiols in water and its applications to a molecular logic gate and living cell imaging.ORGANIC & BIOMOLECULAR CHEMISTRY,15(19),4115-4121.
MLA	Fu, Zhen-Hai,et al."A fluorescein- based chemosensor for relay fluorescence recognition of Cu(II) ions and biothiols in water and its applications to a molecular logic gate and living cell imaging".ORGANIC & BIOMOLECULAR CHEMISTRY 15.19(2017):4115-4121.