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Co-occurrence of nitrite-dependent anaerobic methane oxidizing and anaerobic ammonia oxidizing bacteria in two Qinghai-Tibetan saline lakes
Yang, Jian1,4; Jiang, Hongchen1; Wu, Geng1; Hou, Weiguo2; Sun, Yongjuan3; Lai, Zhongping4; Dong, Hailiang1,5
2012-12-01
发表期刊FRONTIERS OF EARTH SCIENCE
卷号6期号:4页码:383-391
关键词Anammox N-damo Co-occurrence Saline Lakes Qinghai-tibetan Plateau
摘要Nitrite-dependent anaerobic methane-oxidizing (n-damo) bacteria and anaerobic ammonia oxidizing (anammox) bacteria are two groups of microorganisms involved in global carbon and nitrogen cycling. In order to test whether the n-damo and anammox bacteria co-occur in natural saline environments, the DNA and cDNA samples obtained from the surficial sediments of two saline lakes (with salinity of 32 and 84 g/L, respectively) on the Tibetan Plateau were PCR-amplified with the use of anammox- and n-damo-specific primer sets, followed by clone library construction and phylogenetic analysis. DNA and cDNA-based clones affiliated with n-damo and anammox bacteria were successfully retrieved from the two samples, indicating that these two groups of bacteria can co-occur in natural saline environments with salinity as high as 84 g/L. Our finding has great implications for our understanding of the global carbon and nitrogen cycle in nature.
语种英语
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文献类型期刊论文
条目标识符http://ir.isl.ac.cn/handle/363002/5556
专题青海盐湖研究所知识仓储
中国科学院青海盐湖研究所
作者单位1.China Univ Geosci, State Key Lab Biogeol & Environm Geol, Wuhan 430074, Peoples R China
2.China Univ Geosci, State Key Lab Biogeol & Environm Geol, Beijing 100083, Peoples R China
3.Chinese Acad Sci, Qinghai Inst Salt Lakes, Xining 810008, Peoples R China
4.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, State Key Lab Cryospher Sci, Lanzhou 730000, Peoples R China
5.Miami Univ, Dept Geol & Environm Earth Sci, Oxford, OH 45056 USA
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Yang, Jian,Jiang, Hongchen,Wu, Geng,et al. Co-occurrence of nitrite-dependent anaerobic methane oxidizing and anaerobic ammonia oxidizing bacteria in two Qinghai-Tibetan saline lakes[J]. FRONTIERS OF EARTH SCIENCE,2012,6(4):383-391.
APA Yang, Jian.,Jiang, Hongchen.,Wu, Geng.,Hou, Weiguo.,Sun, Yongjuan.,...&Dong, Hailiang.(2012).Co-occurrence of nitrite-dependent anaerobic methane oxidizing and anaerobic ammonia oxidizing bacteria in two Qinghai-Tibetan saline lakes.FRONTIERS OF EARTH SCIENCE,6(4),383-391.
MLA Yang, Jian,et al."Co-occurrence of nitrite-dependent anaerobic methane oxidizing and anaerobic ammonia oxidizing bacteria in two Qinghai-Tibetan saline lakes".FRONTIERS OF EARTH SCIENCE 6.4(2012):383-391.
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