LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization

ISL OpenIR > 青海盐湖研究所知识仓储

	LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization
	Sun, Jing 1; Li, Faqiang2 ; Xu, Wenhua 1; Zhou, Guoying 1; You, Jinmao 1,3; Chen, Guichen 1
	2009-12-01
发表期刊	CHROMATOGRAPHIA
卷号	70 期号:11-12 页码:1627-1633
关键词	Column Liquid Chromatography Lc-esi-ms Free Amino Acids Tibetan Medicine Gentiana Dahurica
摘要	Concentrations of 20 free amino aicds (FAAs) in a famous Tibetan medicine Gentiana dahurica was first investigated using 1,2-benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (BCEOC) as the pre-column fluorescence derivatization reagent by reversed-phase high performance liquid chromatography (RP-LC). 20 amino acid derivatives (AAD) were separated on a Hypersil BDS C(18) column with a good baseline resolution within 65 min. Identification of 20 AAD was by online post-column mass spectrometry with an electrospray ionization (ESI) source. The validation of the method was examined by linearity, repeatability, and detection limits. Most linear correlation coefficients for AAD were > 0.9990, and detection limits (at signal-to-noise of 3:1) were 6.5-178.2 fmol. There were 18 FAAs found in G. dahurica, of which seven FAAs were necessary to the people's health and related to the treatment of liver and gall disease. Variation of concentrations of the 20 FAAs showed geographical distribution difference among populations. Meanwhile a stable genetic diversity of FAAs composition of G. dahurica was also revealed at the species level. Results of the present study proved that the established method was rapid and reproducible for further separation and determination of FAAs in more medicinal plants.
语种	英语
引用统计
文献类型	期刊论文
条目标识符	http://ir.isl.ac.cn/handle/363002/5908
专题	青海盐湖研究所知识仓储盐湖资源与化学实验室
作者单位	1.Chinese Acad Sci, Res Ctr Natl Tibetan Med, NW Inst Plateau Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Qinghai Inst Salt Lakes, Xining 810008, Peoples R China 3.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China
推荐引用方式 GB/T 7714	Sun, Jing,Li, Faqiang,Xu, Wenhua,et al. LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization[J]. CHROMATOGRAPHIA,2009,70(11-12):1627-1633.
APA	Sun, Jing,Li, Faqiang,Xu, Wenhua,Zhou, Guoying,You, Jinmao,&Chen, Guichen.(2009).LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization.CHROMATOGRAPHIA,70(11-12),1627-1633.
MLA	Sun, Jing,et al."LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization".CHROMATOGRAPHIA 70.11-12(2009):1627-1633.