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LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization
Sun, Jing1; Li, Faqiang2; Xu, Wenhua1; Zhou, Guoying1; You, Jinmao1,3; Chen, Guichen1
2009-12-01
Source PublicationCHROMATOGRAPHIA
Volume70Issue:11-12Pages:1627-1633
SubtypeArticle
AbstractConcentrations of 20 free amino aicds (FAAs) in a famous Tibetan medicine Gentiana dahurica was first investigated using 1,2-benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (BCEOC) as the pre-column fluorescence derivatization reagent by reversed-phase high performance liquid chromatography (RP-LC). 20 amino acid derivatives (AAD) were separated on a Hypersil BDS C(18) column with a good baseline resolution within 65 min. Identification of 20 AAD was by online post-column mass spectrometry with an electrospray ionization (ESI) source. The validation of the method was examined by linearity, repeatability, and detection limits. Most linear correlation coefficients for AAD were > 0.9990, and detection limits (at signal-to-noise of 3:1) were 6.5-178.2 fmol. There were 18 FAAs found in G. dahurica, of which seven FAAs were necessary to the people's health and related to the treatment of liver and gall disease. Variation of concentrations of the 20 FAAs showed geographical distribution difference among populations. Meanwhile a stable genetic diversity of FAAs composition of G. dahurica was also revealed at the species level. Results of the present study proved that the established method was rapid and reproducible for further separation and determination of FAAs in more medicinal plants.
KeywordColumn Liquid Chromatography Lc-esi-ms Free Amino Acids Tibetan Medicine Gentiana Dahurica
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; CHLOROFORMATE
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000272173800016
Citation statistics
Document Type期刊论文
Identifierhttp://ir.isl.ac.cn/handle/363002/5908
Collection青海盐湖研究所知识仓储
盐湖资源与化学实验室
Affiliation1.Chinese Acad Sci, Res Ctr Natl Tibetan Med, NW Inst Plateau Biol, Xining 810001, Peoples R China
2.Chinese Acad Sci, Qinghai Inst Salt Lakes, Xining 810008, Peoples R China
3.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China
Recommended Citation
GB/T 7714
Sun, Jing,Li, Faqiang,Xu, Wenhua,et al. LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization[J]. CHROMATOGRAPHIA,2009,70(11-12):1627-1633.
APA Sun, Jing,Li, Faqiang,Xu, Wenhua,Zhou, Guoying,You, Jinmao,&Chen, Guichen.(2009).LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization.CHROMATOGRAPHIA,70(11-12),1627-1633.
MLA Sun, Jing,et al."LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization".CHROMATOGRAPHIA 70.11-12(2009):1627-1633.
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