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LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization
Sun, Jing1; Li, Faqiang2; Xu, Wenhua1; Zhou, Guoying1; You, Jinmao1,3; Chen, Guichen1
2009-12-01
发表期刊CHROMATOGRAPHIA
卷号70期号:11-12页码:1627-1633
文章类型Article
摘要Concentrations of 20 free amino aicds (FAAs) in a famous Tibetan medicine Gentiana dahurica was first investigated using 1,2-benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (BCEOC) as the pre-column fluorescence derivatization reagent by reversed-phase high performance liquid chromatography (RP-LC). 20 amino acid derivatives (AAD) were separated on a Hypersil BDS C(18) column with a good baseline resolution within 65 min. Identification of 20 AAD was by online post-column mass spectrometry with an electrospray ionization (ESI) source. The validation of the method was examined by linearity, repeatability, and detection limits. Most linear correlation coefficients for AAD were > 0.9990, and detection limits (at signal-to-noise of 3:1) were 6.5-178.2 fmol. There were 18 FAAs found in G. dahurica, of which seven FAAs were necessary to the people's health and related to the treatment of liver and gall disease. Variation of concentrations of the 20 FAAs showed geographical distribution difference among populations. Meanwhile a stable genetic diversity of FAAs composition of G. dahurica was also revealed at the species level. Results of the present study proved that the established method was rapid and reproducible for further separation and determination of FAAs in more medicinal plants.
关键词Column Liquid Chromatography Lc-esi-ms Free Amino Acids Tibetan Medicine Gentiana Dahurica
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; CHLOROFORMATE
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS记录号WOS:000272173800016
引用统计
文献类型期刊论文
条目标识符http://ir.isl.ac.cn/handle/363002/5908
专题青海盐湖研究所知识仓储
盐湖资源与化学实验室
作者单位1.Chinese Acad Sci, Res Ctr Natl Tibetan Med, NW Inst Plateau Biol, Xining 810001, Peoples R China
2.Chinese Acad Sci, Qinghai Inst Salt Lakes, Xining 810008, Peoples R China
3.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China
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GB/T 7714
Sun, Jing,Li, Faqiang,Xu, Wenhua,et al. LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization[J]. CHROMATOGRAPHIA,2009,70(11-12):1627-1633.
APA Sun, Jing,Li, Faqiang,Xu, Wenhua,Zhou, Guoying,You, Jinmao,&Chen, Guichen.(2009).LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization.CHROMATOGRAPHIA,70(11-12),1627-1633.
MLA Sun, Jing,et al."LC-ESI-MS Determination of 20 Free Amino Acids in Tibetan Medicine Gentiana dahurica with Pre-Column Fluorescence Derivatization".CHROMATOGRAPHIA 70.11-12(2009):1627-1633.
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