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Inhibition of two gastric cancer cell lines induced by fucoxanthin involves downregulation of Mcl-1 and STAT3
Yu, Rui-xue1; Yu, Rui-tao2,4; Liu, Zhong3
2018
发表期刊HUMAN CELL
卷号31期号:1页码:50-63
文章类型Article
摘要Fucoxanthin is a natural carotenoid that had never been previously demonstrated to have anti-tumor effect on human gastric adenocarcinoma SGC-7901 or BGC-823 cells. Here it was found to inhibit proliferation and induce apoptosis through JAK/STAT signal pathway in these cells; the mechanism by which this occurred was investigated. We find that fucoxanthin significantly increased the number of apoptotic cells by propidium iodide (PI) dye staining and flow cytometry. Fucoxanthin (50 or 75 mu M) induced SGC-7901 cells cycle arrest at S phase, while BGC-823 cells arrest at G2/M phase. RT-PCR and western blot analysis revealed that the expressions of Mcl-1, STAT3 and p-STAT3 were obviously decreased by fucoxanthin in a dose-dependent manner. Synthetic siRNA targeting Mcl-1 was transfected into cells which had no effect on expressions of STAT3. After pretreatment with AG490 (50 mu M) which led to blocking of the JAK/STAT signal pathway, the reductive expressions of Mcl-1, STAT3 and p-STAT3 caused by fucoxanthin were inhibited. This is the first analysis of effects on SGC-7901 and BGC-823 cells by fucoxanthin. Fucoxanthin can induce cell-cycle arrest and apoptosis in these cells. These effects involved downregulation of Mcl-1, STAT3 and p-STAT3. This work is significant for better understanding of mechanisms leading to human gastric adenocarcinoma formation and informing exploitation of anti-tumor marine drug, and for providing Mcl-1 and STAT3 as potential therapeutic targets for gastric adenocarcinoma.
关键词Fucoxanthin Gastric Adenocarcinoma Mcl-1 Stat3
WOS标题词Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]PATHWAY ; EXPRESSION ; APOPTOSIS ; SRC ; INFLAMMATION
收录类别SCI
语种英语
WOS研究方向Cell Biology
WOS类目Cell Biology
WOS记录号WOS:000418841800007
引用统计
文献类型期刊论文
条目标识符http://ir.isl.ac.cn/handle/363002/6411
专题青海盐湖研究所知识仓储
盐湖资源与化学实验室
作者单位1.Pingdingshan Univ, Pingdingshan 467000, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China
3.Chinese Acad Sci, Qinghai Inst Salt Lake, Xining 810008, Qinghai, Peoples R China
4.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Tibetan Med, Xining 810008, Qinghai, Peoples R China
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Yu, Rui-xue,Yu, Rui-tao,Liu, Zhong. Inhibition of two gastric cancer cell lines induced by fucoxanthin involves downregulation of Mcl-1 and STAT3[J]. HUMAN CELL,2018,31(1):50-63.
APA Yu, Rui-xue,Yu, Rui-tao,&Liu, Zhong.(2018).Inhibition of two gastric cancer cell lines induced by fucoxanthin involves downregulation of Mcl-1 and STAT3.HUMAN CELL,31(1),50-63.
MLA Yu, Rui-xue,et al."Inhibition of two gastric cancer cell lines induced by fucoxanthin involves downregulation of Mcl-1 and STAT3".HUMAN CELL 31.1(2018):50-63.
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