ISL OpenIR  > 青海盐湖研究所知识仓储
Inhibition of two gastric cancer cell lines induced by fucoxanthin involves downregulation of Mcl-1 and STAT3
Yu, Rui-xue1; Yu, Rui-tao2,4; Liu, Zhong3
2018
Source PublicationHUMAN CELL
Volume31Issue:1Pages:50-63
SubtypeArticle
AbstractFucoxanthin is a natural carotenoid that had never been previously demonstrated to have anti-tumor effect on human gastric adenocarcinoma SGC-7901 or BGC-823 cells. Here it was found to inhibit proliferation and induce apoptosis through JAK/STAT signal pathway in these cells; the mechanism by which this occurred was investigated. We find that fucoxanthin significantly increased the number of apoptotic cells by propidium iodide (PI) dye staining and flow cytometry. Fucoxanthin (50 or 75 mu M) induced SGC-7901 cells cycle arrest at S phase, while BGC-823 cells arrest at G2/M phase. RT-PCR and western blot analysis revealed that the expressions of Mcl-1, STAT3 and p-STAT3 were obviously decreased by fucoxanthin in a dose-dependent manner. Synthetic siRNA targeting Mcl-1 was transfected into cells which had no effect on expressions of STAT3. After pretreatment with AG490 (50 mu M) which led to blocking of the JAK/STAT signal pathway, the reductive expressions of Mcl-1, STAT3 and p-STAT3 caused by fucoxanthin were inhibited. This is the first analysis of effects on SGC-7901 and BGC-823 cells by fucoxanthin. Fucoxanthin can induce cell-cycle arrest and apoptosis in these cells. These effects involved downregulation of Mcl-1, STAT3 and p-STAT3. This work is significant for better understanding of mechanisms leading to human gastric adenocarcinoma formation and informing exploitation of anti-tumor marine drug, and for providing Mcl-1 and STAT3 as potential therapeutic targets for gastric adenocarcinoma.
KeywordFucoxanthin Gastric Adenocarcinoma Mcl-1 Stat3
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS KeywordPATHWAY ; EXPRESSION ; APOPTOSIS ; SRC ; INFLAMMATION
Indexed BySCI
Language英语
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000418841800007
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.isl.ac.cn/handle/363002/6411
Collection青海盐湖研究所知识仓储
盐湖资源与化学实验室
Affiliation1.Pingdingshan Univ, Pingdingshan 467000, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China
3.Chinese Acad Sci, Qinghai Inst Salt Lake, Xining 810008, Qinghai, Peoples R China
4.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Tibetan Med, Xining 810008, Qinghai, Peoples R China
Recommended Citation
GB/T 7714
Yu, Rui-xue,Yu, Rui-tao,Liu, Zhong. Inhibition of two gastric cancer cell lines induced by fucoxanthin involves downregulation of Mcl-1 and STAT3[J]. HUMAN CELL,2018,31(1):50-63.
APA Yu, Rui-xue,Yu, Rui-tao,&Liu, Zhong.(2018).Inhibition of two gastric cancer cell lines induced by fucoxanthin involves downregulation of Mcl-1 and STAT3.HUMAN CELL,31(1),50-63.
MLA Yu, Rui-xue,et al."Inhibition of two gastric cancer cell lines induced by fucoxanthin involves downregulation of Mcl-1 and STAT3".HUMAN CELL 31.1(2018):50-63.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yu, Rui-xue]'s Articles
[Yu, Rui-tao]'s Articles
[Liu, Zhong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yu, Rui-xue]'s Articles
[Yu, Rui-tao]'s Articles
[Liu, Zhong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yu, Rui-xue]'s Articles
[Yu, Rui-tao]'s Articles
[Liu, Zhong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.