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Subcellular fluorescence imaging for BHK cell and multiple sensing based on carbon dots with two strong emission peaks
Li, Bo1,2; Gong, Deyan1; Li, Xinran1; Zhang, Lilan1; Dong, Yaping2; Li, Wu2; Liu, Weisheng1; Qin, Wenwu1; Wang, Yabin2,3
2018-04-01
发表期刊SENSORS AND ACTUATORS B-CHEMICAL
卷号258页码:757-765
文章类型Article
摘要We develop a novel kind of nitrogen-doped carbon dots (N-CDs) with unprecedented properties through hydrothermal treatment of soluble starch and ethylenediamine. Such N-CDs can emit two strong emission peaks at about 450 nm and 540 nm under a single-wavelength ultraviolet-light excitation. The intensity ratios of the two emission peaks are influenced by pH values, leading to the change of fluorescent colours, which can be utilized to detect solution pH by the change of fluorescent colours. Most importantly, N-CDs can act as fluorescence probes for labelling cell nucleus of BHK cells selectively, indicating that the as-prepared N-CDs have specific targeting towards nucleus cells. In addition, the fluorescence intensity can be quenched by Fe3+ ion and restored by ascorbic acid effectively. The detection limits for Fe3+ and AA were 84.9 nM and 62.1 nM, respectively. (c) 2017 Elsevier B.V. All rights reserved.
关键词Carbon Dots Subcellular Fluorescence Imaging On-off-on Multiple Sensing
WOS标题词Science & Technology ; Physical Sciences ; Technology
关键词[WOS]GRAPHENE QUANTUM DOTS ; ONE-POT SYNTHESIS ; ASCORBIC-ACID ; LIVING CELLS ; BIOLOGICAL APPLICATIONS ; MAGNETIC NANOPARTICLES ; SURFACE-STATES ; IN-VIVO ; NANODOTS ; PH
收录类别SCI
语种英语
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS类目Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS记录号WOS:000424868700087
引用统计
文献类型期刊论文
条目标识符http://ir.isl.ac.cn/handle/363002/6421
专题青海盐湖研究所知识仓储
盐湖资源综合利用工程技术中心
作者单位1.Lanzhou Univ, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Gansu, Peoples R China
2.Chinese Acad Sci, Qinghai Inst Salt Lakes, Xining 810008, Qinghai, Peoples R China
3.Harbin Inst Technol, Sch Chem & Chem Engn, Harbin 150001, Heilongjiang, Peoples R China
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Li, Bo,Gong, Deyan,Li, Xinran,et al. Subcellular fluorescence imaging for BHK cell and multiple sensing based on carbon dots with two strong emission peaks[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,258:757-765.
APA Li, Bo.,Gong, Deyan.,Li, Xinran.,Zhang, Lilan.,Dong, Yaping.,...&Wang, Yabin.(2018).Subcellular fluorescence imaging for BHK cell and multiple sensing based on carbon dots with two strong emission peaks.SENSORS AND ACTUATORS B-CHEMICAL,258,757-765.
MLA Li, Bo,et al."Subcellular fluorescence imaging for BHK cell and multiple sensing based on carbon dots with two strong emission peaks".SENSORS AND ACTUATORS B-CHEMICAL 258(2018):757-765.
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